PARPs-DB Project



About The PARPs Project

We herein describe the architecture and major features of a web-based utility called “PARPs database” (PARPs-DB), which is designed to rationally organize the protein and peptide data generated by the LC-MS/MS analysis of tryptic digest of proteins that co-immunoprecipitate with PARPs proteins into reports meaningful to biological researchers. PARPs-DB offers a LIMS work environment to annotate and study protein-protein interactions and its easy-to-use relational data management system can rapidly supply information pertaining to the biological characteristics of a majority of proteins in a proteomic dataset. The major features of our LIMS are flexibility, compactness, and connectivity to public databases.




Project on our Research Labs


The focus of our laboratory is the study of the action of poly(ADP-ribose) polymerases (PARPs) and their role in the cell. Poly(ADP-ribosylation) is a post-translational protein modification consisting of long chains of poly(ADP-ribose) (pADPr) synthesized by PARPs at the expenses of NAD+. Poly(ADP-ribose) chains are short-lived owing to the activity of the poly(ADP-ribose) glycohydrolase enzyme, which catabolizes the pADPr within minutes of its synthesis15. The PARP family may comprise as many as 17 members which share a common catalytic domain responsible for the synthesis of poly(ADP-ribose)16-18. The best characterized and abundant member of this family is PARP-1, a 113-kDa nuclear protein comprising a DNA-binding domain made of two zinc fingers that allow PARP-1 to be rapidly activated in response to DNA damage. Poly(ADP-ribose) crucially contributes to chromatin remodelling, DNA damage repair, regulation of transcription, and cell division19-21; and PARP-1 is an important actor in many key cellular processes, including BER, transcription, and apoptosis.